DTI Uracil DNA Glycosylase(UNG), Heat-labile, (Glycerol Free) (25 U/ μl) hydrolyzes N-glycosylic bonds between the deoxyribose sugars and the uracil bases in uracil-containing DNA leaving apyrimidinic sites in the DNA. UNG excises uracil from both single- and double-stranded dU-containing DNA but not from RNA. The high concentration glycerol free version makes the product suitable for lyophilisation.
| Brand | Catalogue Number | Pack Size |
|---|---|---|
|
DTI UNG 25 U/µl – Glycerol Free |
DT0106.250 |
250 U |
| Component | Pack Size |
|---|---|
|
DTI Uracil DNA Glycosylase (UNG), Heat-labile, (Glycerol Free) (25 U/μl) |
10 µl |
|
Customized Pack Size |
Available upon request |
| Component | Concentration |
|---|---|
|
Tris-HCl, pH 8.0 (at 4℃) |
20 mM |
|
KCl |
100 mM |
|
DTT |
1 mM |
|
EDTA |
0.1 mM |
|
NP-40 |
0.5% (v/v) |
|
Tween 20 |
0.5% (v/v) |
Storage and Shipment Conditions: -80°C
Source: Produced in E. coli strain expressing a recombinant Xiphophorus maculatus UNG mutant.
Unit Definition: One unit of UNG is defined as the amount of enzyme required to digest 1 μg of uracil-containing dsDNA at 25℃ in 30 min.
Application: Used in further manufacturing and lyophilization processes for PCR, RT-PCR, and LAMP assays.
Inactivation by Heat: The enzyme is completely and irreversibly inactivated by heat incubating at 50℃ for 10 min.
Usage Protocol
• Use as per the requirement. Can be diluted if needed.
• Add suitable enzyme amounts based on application (typically 0.1 – 1 U/50μl reaction volume).
• UNG activation: Incubate for 10 min at 25℃.
• UNG inactivation: Incubate for 2 min at 95℃.
• Follow the specific protocol for application.
Quality Control Data: Please refer to the certificate of analysis for each lot.
| Document | Download Link |
|---|---|
|
DTI UNG 25 U/µl – Glycerol Free – Protocol |
|
|
DTI UNG 25 U/µl – Glycerol Free – Certificate of Analysis |
qPCR reactions was performed with two qPCR reaction mix: One with UNG at a concentration of 2 U/ μl and other without UNG. Both these mixes were spiked with dU-containing amplicons, mimicking carryover contamination. The reaction mix without UNG generated a regular amplification curve, while the reaction mix containing UNG degraded the dU-containing amplicons resulting in significant reduction in amplification from the mimicking carryover contamination.