| Brand | Catalogue Number | Pack Size |
|---|---|---|
|
DTI High Conc. T4 DNA Ligase (2000 U/μl) |
DT1101.40K |
40K U |
Storage and Shipment Conditions: -20°C
| Component | Pack Size |
|---|---|
|
DTI High Conc. T4 DNA Ligase 2000 U/µl |
20 µl |
|
5X Ligation Reaction Buffer |
1 ml |
|
* Customized pack size available |
| Component | Concentration |
|---|---|
|
Tris-HCl, pH 7.6 |
330 mM |
|
MgCl2 |
50 mM |
|
DTT |
5 mM |
|
ATP |
5 mM |
|
PEG#6000 |
37.5% |
Source: Escherichia coli carrying the plasmid that encodes the gene of T4 DNA ligase.
Unit Definition: One unit is the amount of enzyme that ligates more than 90% of 6 μg of λDNA-Hind III fragments in a 20 μl mixture in 30 minutes at 16°C. One unit of this enzyme corresponds to 0.008 Weiss units by the ATP-PPi exchange reaction in 66 mM Tris-HCl, pH 7.6 that contains 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, and 3.3 μM [32P]-Na4P2O7.
Applications:
| Document | Download Link |
|---|---|
|
DTI High Conc. T4 DNA Ligase 2000 U/µl – Protocol |
|
|
DTI High Conc. T4 DNA Ligase 2000 U/µl – Certificate of Analysis |
Efficient ligation of λ HIND III DNA obtained using the enzyme and buffer.
Band of 2027 bp was clearly identified by agarose gel electrophoresis. The same image is in the right side.
Figure 1: Efficient ligation of λ HIND III DNA with a 2027 bp band clearly identified by agarose gel electrophoresis.