DTI FabTaq HS DNA Polymerase is the hot-start version of our high-performing DTI FabTaq polymerase offering high yield, excellent sensitivity, and fidelity that is 4.5 times higher than Taq polymerase. Antibody-mediated hot-start gives lower background, higher specificity, and allows room temperature reaction assembly. DTI FabTaq HS DNA polymerase is supplied with 10X buffer (Mg2+) and dNTP mix.
| Catalog # | Pack Size |
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DT0102.250 |
250 U
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DTI CobaltAmp HiFid Taq HS Premix is a Fast PCR Master Mix and designed for streamlined PCR, and is, therefore, ideal for applications such as colony PCR. It contains a hot-start PCR enzyme, optimized buffer, dNTPs, along with a blue gel-loading dye and density reagent, in a convenient 2X premix format. Reactions are assembled by simply mixing primers and DNA template with DTI CobaltAmp HiFid Taq HS Premix. For rapid and convenient screening, the amplification reactions can be loaded directly onto an agarose gel immediately after amplification without purification, or digested directly with restriction enzymes.
Antibody-mediated hot start
Lower background, increased specificity, and room-temperature reaction assembly.
Low bacterial DNA contamination
Helps reduce contamination in sensitive experiments.
Premix Taq
Enables easy PCR setup and minimizes pipetting steps for convenience.
| Document | Download Link |
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DTI FabTaq HS – Protocol |
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DTI FabTaq HS – Certificate of Analysis |
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DTI FabTaq HS – Promotional Document |
Comparison with DTI FabTaq HS DNA polymerase
The performance of DTI FabTaq HS in a multiplex reaction was compared to its performance in single-target reactions, and to that of the standard DTI FabTaq DNA Polymerase in a multiplex reaction (Figure 1). DTI FabTaq and DTI FabTaq HS were each used to amplify a human genomic DNA template with eight different primer pairs, each specific for a target ranging from 84 to 432 bp in size. In addition, DTI FabTaq HS was used to perform an individual amplification reaction with each of the eight primer pairs.
The sample amplified using DTI FabTaq HS (Lane 10) shows more efficient multiplex amplification of all the bands individually amplified in Lanes 1–8 than the sample amplified using DTI FabTaq (Lane 9), and it lacks the low molecular weight non-specific amplification band seen with DTI FabTaq (Lane 9). The intensities of the bands in Lane 10 are comparable to the intensities of the corresponding individual bands in Lanes 1–8, indicating that DTI FabTaq HS provides multiplex efficiencies comparable to those observed in individual amplification reactions.
Comparing the ability of DTI FabTaq DNA Polymerase Hot Start Version to amplify human genomic DNA fragments to that of the standard DTI FabTaq DNA Polymerase. PCR reactions were performed using human genomic DNA as a template and primer pairs for eight different targets. Lanes 1–8 contain individual reactions for each primer pair amplified using DTI FabTaq HS. Lanes 9 and 10 contain multiplex PCR reactions performed with all eight primer pairs in a single tube, amplified with either the standard Taq DNA polymerase (DTI FabTaq, Lane 9) or DTI FabTaq HS (Lane 10).
Conclusions
This experiment demonstrates that target amplification efficiencies for multiplex PCR using DTI FabTaq HS are comparable to efficiencies observed for separate (single target) amplification reactions. In addition, DTI FabTaq HS demonstrates superior efficiency and specificity over standard Taq polymerase (DTI FabTaq) in this multiplex-PCR application.
Methods
PCR cycling conditions for individual reactions:
1 cycle at 94°C for 30 sec, followed by 30 cycles of 94°C for 30 sec, 55°C for 30 sec, and 72°C for 60 sec.
PCR cycling conditions for multiplex reactions:
1 cycle at 94°C for 30 sec, followed by 30 cycles of 94°C for 30 sec, 57°C for 30 sec, and 72°C for 60 sec, followed by a final step at 72°C for 90 sec.
Figure 1: Comparison of DTI FabTaq and DTI FabTaq HS DNA polymerases in multiplex PCR reactions.
Comparing the ability of DTI FabTaq DNA Polymerase Hot Start Version to amplify human genomic DNA fragments to that of the standard DTI FabTaq DNA Polymerase. PCR reactions were performed using human genomic DNA as a template and primer pairs for eight different targets. Lanes 1–8 contain individual reactions for each primer pair amplified using DTI FabTaq HS. Lanes 9 and 10 contain multiplex PCR reactions performed with all eight primer pairs in a single tube, amplified with either the standard Taq DNA polymerase (DTI FabTaq, Lane 9) or DTI FabTaq HS (Lane 10).
Conclusions
This experiment demonstrates that target amplification efficiencies for multiplex PCR using DTI FabTaq HS are comparable to efficiencies observed for separate (single target) amplification reactions. In addition, DTI FabTaq HS demonstrates superior efficiency and specificity over standard Taq polymerase (DTI FabTaq) in this multiplex-PCR application.
Methods
PCR cycling conditions for individual reactions:
1 cycle at 94°C for 30 sec, followed by 30 cycles of 94°C for 30 sec, 55°C for 30 sec, and 72°C for 60 sec.
PCR cycling conditions for multiplex reactions:
1 cycle at 94°C for 30 sec, followed by 30 cycles of 94°C for 30 sec, 57°C for 30 sec, and 72°C for 60 sec, followed by a final step at 72°C for 90 sec.