DTI Bca DNA Polymerase- Glycerol Free originates from a thermophilic bacterium Bacillus caldotenax, that lacks 5’ → 3’ exonuclease activity. By introducing mutations into the protein, the activity of both DNA polymerase and reverse transcriptase is highly enhanced while exerting the original strong strand-displacement activity. Therefore, the isothermal amplifications using this enzyme enable to amplify the target product from a little nucleic acid in a shorter time. This enzyme is originally isolated and developed independently by Takara Bio Inc, Japan. DTI manufactures and sells it under an agreement with Takara Bio Inc.
| Brand | Catalogue Number | Pack Size |
|---|---|---|
|
DTI Bca DNA Polymerase- Glycerol Free (8U/μl) |
DT0104.80 |
80 U |
| Component | Pack Size |
|---|---|
|
DTI Bca DNA Polymerase- Glycerol Free (8U/μl) |
10 µl |
|
Customized Pack Size |
Overview:
Storage and Shipment Conditions: -80°C
Materials required but not provided:
Source: Escherichia coli carrying a plasmid containing the gene for BCA DNA Polymerase
Unit definition: One unit is defined as the amount of enzyme that incorporates 210 nmol of dNTP into acid-insoluble precipitate in 30 minute at 65°C.
Application:
• LAMP (Loop-Mediated Isothermal Amplification)
• RCA (Rolling Circle Amplification)
Precautions for use:
• Don’t mix the enzyme vigorously.
• Extreme cautions should be taken during reaction preparation to avoid contamination. If analyzing the products after reaction, execute in a different area from the reaction preparation.
• Reaction temperatures above 70℃ are not recommended. Hence this enzyme can’t be used for conventional PCR.
| Document | Download Link |
|---|---|
|
DTI Bca DNA Polymerase – Glycerol Free – Protocol |
|
|
DTI Bca DNA Polymerase – Glycerol Free – Certificate of Analysis |
Experimental Sample
1. LAMP assay: Target gene was detected using 0.01 – 0.01 ng of E. coli genomic DNA when the isothermal amplification was performed using the enzyme. The protocol used was: Incubate at 63℃ for 90 minutes.
Figure 1: LAMP assay showing target gene detection using E. coli genomic DNA.
2. RT-LAMP assay: Target gene was detected using 0.01 – 0.01 ng of human total RNA when the RT-isothermal amplification was performed using AMV reverse transcriptase (Takara cat# 2630A) and Bca DNA polymerase. The protocol used was: Incubate at 63℃ for 90 minutes.
Figure 2: RT-LAMP assay showing target gene detection using human total RNA.