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TaKaRaTaq™ (rTaq DNA Polymerase without dNTPs)

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TaKaRaTaq™ (rTaq DNA Polymerase without dNTPs)

Takara Taq DNA Polymerase is a recombinant version of full-length Taq polymerase. It has the same characteristics and capabilities as the native Taq polymerase, and is suitable for a variety of standard PCR applications. The polymerase is supplied with separate tube of buffer (Mg2+ plus).
Brand Catalogue Number Pack Size
Takara Taq (r Taq DNA Polymerase without dNTPs)
R500A
250 Units
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Takara Taq (r Taq DNA Polymerase without dNTPs)

Storage: -20℃

Applications:

  1. For DNA amplification by PCR-well mini-gel, respectively.
  2. For DNA sequencing

Storage Buffer:

Components Concentration
Tris-HCl (pH 8.0)
20 mM
KCl
100 mM
EDTA
0.1 mM
DTT
1 mM
Tween 20
0.5%
Nonidet P-40
0.5%
Glycerol
50%

Unit Definition: One unit is the amount of enzyme that will incorporate10 nmol of dNTP into acid-insoluble products in 30 minutes at 74℃ with activated salmon sperm DNA as the template-primer.

Purity: Nicking, endonuclease and exonuclease activity were not detected after the incubation of 0.6 μg of supercoiled pBR322 DNA, 0.6 μg of λDNA or 0.6 μg of λ-Hin d III digest with 10 units of this enzyme for 1 hour at 74℃.

Source: Escherichia coli carrying a plasmid that encodes the Thermus aquaticus DNA Polymerase gene.

Takara Taq (r Taq DNA Polymerase without dNTPs)
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Takara Taq (r Taq DNA Polymerase without dNTPs) – Protocol
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