The DTI FabSpin Universal DNA extraction Kit is designed for DNA extraction and purification from a variety of from a variety of sample sources including blood, gram-negative bacteria, mammalian cul¬tured cells, plant tissue and animal tissue. This kit employs a proprietary lysis buffer in com¬bination with Spin Column membrane to efficiently purify genomic DNA from the biological sample. The protocol provides a simple method to achieve the rapid isolation of highly puri¬fied genomic DNA and the entire procedure can be accomplished within 20 minutes after tissue cell lysed. Using the kit about 10 μg of highly purified genomic DNA can be extracted from 50 – 200 μl of mammalian whole blood (with anticoagulant), 1 – 10 μl of nucleated erythrocyte in whole blood (with anticoagulant), 1.0 – 5.0 x 109 gram-negative bacteria, 1.0 x 105 – 1.0 x 107 cultured cells, 2 – 30 mg of mammalian tissues and 25 – 100 mg plant tissues. Genomic DNA prepared by this kit is suitable for a variety of applications, such as PCR, South¬ern blotting, RAPD, AFLP, RFLP and other molecular biology experiments.
| Brand | Catalogue Number | Pack Size |
|---|---|---|
|
DTI Universal DNA Extraction Kit |
DT9701.100 |
100 Preps |
|
DTI Universal DNA Extraction Kit |
DT9701.250 |
250 Preps |
Kit Specifications: DTI Green HiFid Taq HS II Premix is a ready-to-use PCR reagent mix that provides high specificity, sensitivity, and performance for various PCR applications.
High Purity DNA: Obtain genomic DNA with an A260/A280 ratio between 1.8-2.0, suitable for PCR, Southern blotting, RAPD, AFLP, RFLP, and other molecular experiments.
Storage and Shipment: The buffer components should be stored at room temperature (15 – 25°C). Lyophilised Proteinase K should be stored at -20°C for 2 years, 4°C for 18 months, and room temperature for 12 months. Once reconstituted, store at -20°C.
Materials Required: Sterile mortar and pestle, Liquid Nitrogen, 100% ethanol, Sterilized water, Phosphate Buffered Saline (PBS), RNAse A, Centrifuge, Vortex, Water bath (56°C), Micropipettes, Pipette tips, Personnel protection equipment (lab coat, gloves, goggles).
Preparation Before Experiment: Proteinase K preparation, warming Buffer GL if precipitation occurs, preheating Elution Buffer to 65°C for improved elution efficiency, and ethanol preparation for Buffer WB.
| Components | Cat# DT9701.100P (100 Preps) | Cat# DT9701.250P (250 Preps) |
|---|---|---|
|
Proteinase K (Lyophilised) |
40 mg |
100 mg |
|
Proteinase K Reconstitution Buffer |
2 ml |
5 ml |
|
Buffer GL |
24 ml |
60 ml |
|
Buffer GB |
24 ml |
60 ml |
|
Buffer WA |
56 ml |
140 ml |
|
Buffer WB |
48 ml |
120 ml |
|
Elution Buffer |
28 ml |
70 ml |
|
PBS |
20 ml |
50 ml |
|
Nuclease Free Water |
5 ml |
12.5 ml |
|
Spin Columns (Green Ring) |
100 pcs |
250 pcs |
|
Collection Tubes |
100 pcs |
250 pcs |
|
SDS Insert Sheet |
1 |
1 |
|
CoA |
1 |
1 |
| Document | Download Link |
|---|---|
|
DTI Universal DNA Extraction Kit – Protocol |
|
|
DTI Universal DNA Extraction Kit – Certificate of Analysis (25 Preps) |
|
|
DTI Universal DNA Extraction Kit – Certificate of Analysis (100 Preps) |
|
|
DTI Universal DNA Extraction Kit – Certificate of Analysis (250 Preps) |
Figure 1: Electrophoresis image of genomic DNA extracted from fish gill tissue using the DTI FabSpin universal DNA extraction kit.