Agarose electrophoresis is the most effective way to identify and separate DNA fragments. The purity of agarose directly affects the resolution of DNA and the clarity of electrophoresis results. If the agarose contains sugars, salts, proteins, it will affect the migration speed of DNA in the gel and subsequently the reaction performance of recovered DNA fragments during enzymatic reactions. Hence, using high quality agarose is important for success of the experiment. DTI Agarose routine is a high-quality agarose product, suitable for making 1-3% agarose gel. When stained with EtBr, the electrophoretic separation performance is strong, bands are clear and is suitable for electrophoresis of various DNA fragments. It is an economical and ideal grade for conventional agarose electrophoresis.
| Brand | Catalogue Number | Pack Size |
|---|---|---|
|
DTI Agarose Premium |
DT1702.100 |
100 g |
|
DTI Agarose Premium |
DT1702.500 |
500 g |
Gel Strength (1.0% gel): >1200 g/cm²
Gelling temperature: 34.5°-37.5° C
Electroendosmosis: 0.05-0.13
Sulfate content: <0.1%
| Cat. No. | Download Link |
|---|---|
|
DTI Agarose Premium – Protocol |
|
|
DTI Agarose Premium – Certificate of Analysis |
|
|
DTI Agarose Routine – Promotional Document |
Comparative runs of DNA markers on DT1702.500 Agarose premium vs DT1701.500 Agarose Routine.
a) λ Hind III digest DNA Marker run on 0.7% Agarose gel made using TAE buffer
| Lane # | Description | Fragment Size for Reference |
|---|---|---|
|
1,2 |
Cat# 3403, λ Hind III digest DNA Marker |
23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125. The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min. |
b) 100 bp DNA Marker run on 3% Agarose gel made using TAE buffer
| Lane # | Description | Fragment Size for Reference |
|---|---|---|
|
1,2 |
Cat#DT1801.100, DTI 100bp Ladder (Dye Plus) |
1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100 base pairs |