Agarose electrophoresis is the most effective way to identify and separate DNA fragments. The purity of agarose directly affects the resolution of DNA and the clarity of electrophoresis results. If the agarose contains sugars, salts, proteins, it will affect the migration speed of DNA in the gel and subsequently the reaction performance of recovered DNA fragments during enzymatic reactions. Hence, using high quality agarose is important for success of the experiment. DTI Agarose routine is a high-quality agarose product, suitable for making 1-3% agarose gel. When stained with EtBr, the electrophoretic separation performance is strong, bands are clear and is suitable for electrophoresis of various DNA fragments. It is an economical and ideal grade for conventional agarose electrophoresis.
| Brand | Catalogue Number | Pack Size |
|---|---|---|
|
DTI Agarose Routine |
DT1701.500 |
500 g |
Gel Strength (1.5% gel): >800 g/cm²
Gelling temperature: 35°-40° C
Electroendosmosis: 0.09-0.12
Sulfate content: 0.09-0.12
| Cat. No. | Download Link |
|---|---|
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DTI Agarose Routine – Protocol |
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DTI Agarose Routine – Certificate of Analysis |
Experimental sample: The below image indicates the run of different DNA markers as indicated in the table, on a 2% TAE gel.
| Lane # | Description | Fragment Size for Reference |
|---|---|---|
|
1,2 |
Cat#DT1801.100, DTI 100bp Ladder (Dye Plus) |
1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100 base pairs |
|
3,4 |
Cat#DT1803.100, DTI2000 DNA Ladder (Dye Plus) |
2000, 1000, 750, 500, 250, 100 base pairs |
|
5,6 |
Cat#3428A, DL5000 DNA Ladder, Fragment Size (bp) |
5000, 3000, 2000, 1500, 1000, 750, 500, 250, 100 base pairs |
|
7,8 |
Cat#DT1802.100, DTI 1 kb Ladder (Dye Plus) |
10, 6, 5, 4, 3, 2, 1 Kilo base pairs |