Enhancing PCR Efficiency and Accuracy DTI FabSpeed Thermal Cycler is designed specifically to enhance PCR efficiency and accuracy. It is equipped with a 7” sensitive touchscreen and a friendly graphic user interface, which makes operation highly intuitive. With flexible ramp rate and gradient temperature control, DTI FabSpeed greatly increases PCR accurate optimization.
| Brand | Product | Catalogue Number |
|---|---|---|
|
DTI Brand |
DTI FabSpeed Thermal Cycler |
TCST-9622 |
Flexible Ramp Rate Control:
From 0.1 – 5.5 ℃/sec to meet the need for the CRISPR related assays.
Fully Adjustable Lid Temperature:
Can be set between 35 and 120 °C for virtually any type of experiment including NGS pre-treatment.
Gradient Optimization:
Range from 1 to 30 °C enables optimal experimental conditions in a single PCR run.
Sensitive 7” Capacitive Touchscreen:
Enables easy operation even with laboratory gloves.
Graphic Interface:
Easy to use, making the adjustment of experiment parameters such as temperature, time, and cycle quick and simple.
Sample Block:
96 Well, Gradient Block: Compatible with regular profile or low profile 0.2 ml PCR tube, strip, non-skirted, semi-skirted, and full-skirted 96-well plate.
Block Temperature:
• Block Temperature Range: 4.0 – 100 °C
• Max. Heating Rate: 5.5 °C/Sec
• Max. Cooling Rate: 3.3 °C/Sec
• Temperature Accuracy: +/- 0.3 °C
• Temperature Uniformity Across Block: +/- 0.3 °C
• Adjustable Ramp Rate: 0.1 – 5.5 °C/Sec
Gradient Temperature:
• Gradient Direction: Horizontal across the block
• Gradient Temperature Range: 30 – 100 °C
• Gradient Temperature Difference: Max. span 30 °C
Heater Lid:
• Temperature Setting Range: 35 – 120 °C or off
• Temperature Accuracy: +/- 1.0 °C
Software:
• Portability of Protocols: Save and transfer to computer or DTI FabSpeed Thermal Cycler via USB flash drive
• Stored Program No.: > 4000 sets
• Registered User Folder No.: 100 sets
• User Folder Password Protection: Yes
• Run Status Report: Yes, HTML output and transfer via USB flash drive
• Real-time Temp. Profile Export: Yes, CSV output and transfer via USB flash drive
• Tools: Tm calculator, Copy number converter, Master mix preparation wizard
General:
• Display: 7” color LCD with capacitive touch panel
• Data Port: 1 USB Type-A front port for USB flash drive
• Heated Lid: 35 – 120 °C or off
• Auto Restart after Power Outage: Yes
• Footprint Dimensions (H x W x D): 225 mm x 245 mm x 415 mm
• Weight: 9.5 kg
• Power Supply: AC 100-240 V, 50/60 Hz, 750 W
• Certification: CE, RoHS
| Document | Download Link |
|---|---|
|
DTI FabSpeed Thermal Cycler – Operation Manual |
|
|
DTI FabSpeed Thermal Cycler – Promotional Document |
Amplification of Myc gene (4Kb) of Human Genomic DNA is performed using EmeraldAmp GT PCR master mix (Takara brand cat# RR310A). Amplification of IGF2R gene (6Kb) of Human Genomic DNA is performed using EmeraldAmp Max PCR master mix (Takara brand cat# RR320A).
The thermal cycling protocol used was as below:
| Temperature | Duration | Cycles |
|---|---|---|
|
98°C |
10 seconds |
30 Cycles |
|
60°C |
30 seconds |
30 Cycles |
|
72°C |
4 minutes |
30 Cycles |
PCR products are compared using Agarose gel electrophoresis using 1 kb DNA ladder (Takara brand cat# 3426A).
Amplification of β-globin gene (20Kb) of Human Genomic DNA is performed using PrimeStar GXL DNA premix (Takara brand cat# R051).
The thermal cycling protocol used was as below :
| Temperature | Duration | Cycles |
|---|---|---|
|
98°C |
10 seconds |
30 Cycles |
|
68°C |
4 Minutes |
30 Cycles |
Amplification of long DNA fragments that could be challenging can be efficiently performed using DTI FabSpeed Thermal Cycler owing to its high ramp rate feature. The thermal cycler is compatible with DNA polymerases capable of high-speed PCR, including PrimeSTAR GXL DNA Polymerase, as used in the above assay.
