Enhancing PCR Efficiency and Accuracy DTI FabSpeed Thermal Cycler is designed specifically to enhance PCR efficiency and accuracy. It is equipped with a 7” sensitive touchscreen and a friendly graphic user interface, which makes operation highly intuitive. With flexible ramp rate and gradient temperature control, DTI FabSpeed greatly increases PCR accurate optimization.
TCST-9622
Flexible Ramp Rate Control:
From 0.1 – 5.5 ℃/sec to meet the need for the CRISPR related assays.
Fully Adjustable Lid Temperature:
Can be set between 35 and 120 °C for virtually any type of experiment including NGS pre-treatment.
Gradient Optimization:
Range from 1 to 30 °C enables optimal experimental conditions in a single PCR run.
Sensitive 7” Capacitive Touchscreen:
Enables easy operation even with laboratory gloves.
Graphic Interface:
Easy to use, making the adjustment of experiment parameters such as temperature, time, and cycle quick and simple.
| Document | Download Link |
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DTI FabTaq HS-Glycerol Free (50U/ul) – Protocol |
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DTI FabTaq HS-Glycerol Free (50U/ul) – Certificate of Analysis |
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DTI FabTaq HS-Glycerol Free (50U/ul) – Promotional Document |
Experimental Sample
a) Amplification data: Good performance of this product has been confirmed by PCR using λ DNA as template (amplified fragment size 8 kbp). The protocol used for the assay and the results are as follows:
Figure 1: Amplification data showing product performance with λ DNA as template.
b) Hot start function data: Test target sample added to a PCR system containing FabTaq and FabTaq HS respectively. Following an incubation at RT for 30 mins, the products were amplified using a thermal cycler. Non-specific bands (around 280 bp) were observed in the PCR system along with specific target bands (838 bp) using FabTaq, while there were no non-specific bands observed in PCR system using FabTaq HS.
Figure 2: Amplification data for λ DNA (8 kbp amplified fragment size).
Figure 3: Additional data showcasing product performance.
Figure 4: Hot start function data showing non-specific bands in PCR system with FabTaq and absence of non-specific bands with FabTaq HS.